Yale University_Spatial ATAC Sequencing for Fixed Fresh Frozen Human Lymph node Tissue via DBiT-seq v1
Year: 2023
Type: preprint
Abstract: This protocol describes the use of Deterministic Barcoding in Tissue for spatial ATAC sequencing to construct an epigenome atlas of fixed fresh frozen human lymph node tissue samples. This approach uses a microfluidic-based method to introduce combinatorial DNA oligo barcodes directly to the cells in a tissue section fixed on a glass slide. This method spatially resolved genome-wide profiling of transposase-accessible chromatin sequencing for chromatin accessibility.This technique does not directly resolve single cells but can achieve a near-single-cell resolution for spatial epigenomic analysis.
Institution: Yale University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4403636694