Electrophoretic removal of sinapic acid from rapeseed protein extract
Year: 2024
Type: article
Abstract: Isolation of proteins from oilseeds to supply functional proteins for the food industry is essential but challenging due to the presence of anti-nutrients such as phenolic compounds. To deliver proteins, the removal of phenolic compounds is crucial. Conventionally, this is accomplished by alcohol washing; however, this is resource-intensive, may be unacceptable for some and does not provide proteins with good techno-functional properties since it alters the native protein structure. To overcome such drawbacks, gentle processing methods must be developed. In this work, we investigated the electro-separation of sinapic acid from rapeseed protein extract. A porous medium (ion exchange or ultrafiltration membrane) permitting electromigration of only sinapic acid and retaining the proteins was utilized under two different potential differences. The electro-separation of sinapic acid relied on electrostatic and electrophoretic forces, which cause their adsorption and permeation. Among the treatments, 1.5 V over an anion exchange membrane showed the best performance, providing considerable sinapic acid removal (34.0 ± 4.0 wt%) while maintaining the protein content and pH stability. A larger system with a larger membrane surface area yielded as high as 90.3 ± 3.8 wt% of sinapic acid removal within 240 min while retaining 88.8 ± 7.6 wt% of the proteins.
Institution: Wageningen University & Research
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,790
Funder: Milli Eğitim Bakanliği
OpenAlex ID: W4403667751