Exploring the Siderophore Portfolio for Mass Spectrometry-Based Diagnosis of Scedosporiosis and Lomentosporiosis
Year: 2024
Type: article
Abstract: High Resolution Image Download MS PowerPoint Slide Scedosporium apiospermum and Lomentospora prolificans secrete siderophores (iron scavengers) during hyphal proliferation. Siderophores are virulence factors and potential clinical biomarkers of invasive scedosporiosis and lomentosporiosis. Both strains secreted a uniform spectrum of siderophores, including coprogen B (CopB), N α -methyl-coprogen B, dimethyl-coprogen, and ferricrocin, with N α -methyl-coprogen B being the fastest secreted and most abundant coprogen. Under iron and zinc restriction, reflecting a nutrient-limited host environment, L. prolificans secreted 45 times more CopB than did S. apiospermum, presumably contributing to its higher virulence. This robust mobilization of CopB was further enhanced by zinc surplus. Additionally, two novel cyclic peptides, Scedocyclin A and B, were characterized in Scedosporium boydii using the de novo sequencing tool CycloBranch. Utilizing matrix-assisted laser desorption/ionization, the portfolio of coprogens detected had limits of detection and quantitation of 4.9 and 14.6 fmol/spot in complex matrices, respectively, making them strong candidates for the next-generation, routine diagnosis of invasive scedosporiosis and lomentosporiosis through the Biotyper siderotyping.
Source: ACS Omega
Institutions: Czech Academy of Sciences, Czech Academy of Sciences, Institute of Microbiology, Palacký University Olomouc, Czech Technical University in Prague, Charles University, University Hospital in Motol, University of Ostrava, Public Health Institute Ostrava, Stanford Medicine, Stanford University, Inserm, Institut de Recherche en Santé, Environnement et Travail, Centre Hospitalier Universitaire de Rennes
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,935
Funders: Univerzita Karlova v Praze, České Vysoké Učení Technické v Praze, Institut National de la Santé et de la Recherche Médicale, Ministerstvo Školství, Mládeže a Tělovýchovy, Akademie Věd České Republiky, Ostravská Univerzita v Ostravě, Univerzita Palackého v Olomouci, Centre Hospitalier Universitaire de Rennes, School of Medicine, Stanford University, Mikrobiologický Ústav, Akademie Věd České Republiky
Awards: G2340803269, G5007093561
OpenAlex ID: W4403689845