Evaluation of anti-Toxoplasma effects of solid lipid nanoparticles carrying Cinnamon zeylanicum and Moringa oleifera oil extracts
Year: 2024
Type: article
Abstract: The fabrication of anti- Toxoplasma drugs with less side effects and desirable efficacy is one of the important research goals facing with toxoplasmosis. This study aimed to determine the anti- Toxoplasma effects of Cinnamon zeylanicum (CZ), Moringa oleifera (MO) oil encapsulated into solid lipid nanoparticles (SLNs). Vero cells were cultured with serial concentrations (1 mg/mL to 100 µg/mL) of CZ-SLNs and MO-SLNs in DMEM culture medium. The morphological, physical, and chemical features of nanoparticles were calculated. The cell viability assays and anti- T. gondii effects of CZ-SLNs and MO-SLNs were evaluated. The CC 50 and IC 50 indices of SLNs-enveloped extracts were calculated. The particle sizes of MO-SLNs and CZ-SLNs were 411.5 and 365 nm, while PDI indices were 0.53 and 0.7, respectively. Transmission electron microscopy (TEM) showed that both MO-SLNs and CZ-SLNs were smoothed spherical nanoparticles with rounded edges. The cytotoxicity assay showed the CC 50 value of MO-SLNs at concentrations of ˃10 mg/mL. In addition, 60% of T. gondii -infected Vero cells remained alive at the concentrations ≤ 1 mg/ml, while the MO-SLNs killed at least 90% of T. gondii tachyzoites with an IC 50 > 1 µg/ml. The cytotoxicity of CZ-SLNs extract showed the CC 50 at the concentration 0.1 mg/mL. More than 50% of Vero cells, infected with T. gondii tachyzoites, survived at a concentration less than 0.1 mg/mL (IC 50 ˂ 0.1 mg/mL), while the CZ-SLNs killed at least 85% of T. gondii tachyzoites in all concentrations. The current results represented that the use of SLNs as a nano-carrier for M. oleifera and C. zeylanicum could kill T. gondii tachyzoites with low cytotoxicity, suggesting the effectiveness of these nano-emulsions along with the chemical agents in the treatment of Toxoplasma.
Authors: Mahsa Mottaghi, Parisa Karami, Zahra Hesari, Sara Nemati, Hanieh Mohammad Rahimi, Hamed Mirjalali
Institutions: Islamic Azad University, Tehran, Islamic Azad University, Science and Research Branch, Islamic Azad University Central Tehran Branch, Guilan University of Medical Sciences, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University, Shahid Beheshti University of Medical Sciences
Language: English
Subfield: Parasitology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,195
Award: G3345708672
OpenAlex ID: W4403705938