Work

The FSGS protein actinin-4 interacts with NKCC2 to regulate thick ascending limb NaCl reabsorption

Year: 2024

Type: article

Abstract: ACTN4 function and dysfunction in glomerular podocytes have been extensively studied. However, the function of ACTN4 in the nephron has not been studied. Our paper shows for the first time that ACTN4, in the nephron, regulates NaCl reabsorption in part by affecting NKCC2 surface expression. Protein-protein interactions between ACTN4 and NKCC2 seem to mediate NKCC2 endocytosis in TALs. When ACTN4 was silenced in the TAL in vivo using CRISPR/Cas9 or shRNAs, surface NKCC2 and NaCl reabsorption increased.

Source: American Journal of Physiology-Renal Physiology

DOI: 10.1152/ajprenal.00119.2024

Authors: Dipak Maskey, Tang-Dong Liao, D’Anna L. Potter, Pablo A. Ortiz

Institutions: Henry Ford Health, Wayne State University, Henry Ford Hospital

Language: English

Topic: Ion Transport and Channel Regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funders: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases