Work

Two new flavonoids from the leaves of Garcinia smeathmannii, in vitro and in silico anti-inflammatory potentials

Year: 2024

Type: article

Abstract: Garcinia smeathmannii is a well-known plant for its uses in the effective treatment of intestinal parasites, skin eruptions and skin burns. The dichloromethane-methanol (2:3) crude extract of the leaves of G. smeathmannii led to the isolation and characterization of twenty compounds ( 1 − 20 ) using chromatographic and spectroscopic techniques. Extracts and compounds were screened in vitro for their anti-inflammatory (ROS), antiglycation and antileishmanial ( L. tropica ) activities. Compounds were also screened for their in silico anti-inflammatory activities using Maestro 4.2.1 software with the co-crystal complex structures of the ovine oCOX-1: meloxicam (PDB Id: 4O1Z ) and murine mCOX-2: meloxicam (PDB Id: 4M11 ) proteins. An unprecedented flavonol ( 1 ) and a flavone dimer ( 2 ) together with eighteen known compounds ( 3 − 20 ) were characterized. All the tested samples in vitro revealed no antiglycation and antileishmanial activities. Beside, extracts revealed moderate anti-inflammatory activities (IC 50 ranging from 24.1 ± 2.0 to 34.7 ± 0.8 μg/mL). Only compound ( 13 ) revealed an anti-inflammatory activity which was 9.33 times more active than the reference (Ibuprofen, IC 50 = 11.2 ± 1.9 μg/mL) with IC 50 of 1.2 ± 0.0 μg/mL. Compounds ( 2 – 9 , 11 – 13 and 19 – 20 ) were docked and the docking scores were ranging from −10.178 to −6.119 (kcal/mol) which were in agreement with the experimental anti-inflammatory activity. These results are in agreement with the traditional uses of the leave of G. smeathmannii as cataplasm for skin eruption and as analgesic agent. • Phytochemical investigation of Garcinia smeathmannii (Planch. & Triana) Oliv. • Chemophenetic significance of the isolated compounds. • Anti-inflammatory (ROS), antiglycation and antileishmanial ( L. tropica ) screening of samples from G smeathmannii. • Molecular docking of the isolated compounds for anti-inflammatory potencies.

Source: Fitoterapia

DOI: 10.1016/j.fitote.2024.106273