3D Micropatterned Traction Force Microscopy: A Technique to Control 3D Cell Shape While Measuring Cell‐Substrate Force Transmission
Year: 2024
Type: article
Abstract: Cell shape and function are intimately linked, in a way that is mediated by the forces exerted between cells and their environment. The relationship between cell shape and forces has been extensively studied for cells seeded on flat 2D substrates, but not for cells in more physiological 3D settings. Here, a technique called 3D micropatterned traction force microscopy (3D-µTFM) to confine cells in 3D wells of defined shape, while simultaneously measuring the forces transmitted between cells and their microenvironment is demonstrated. This technique is based on the 3D micropatterning of polyacrylamide wells and on the calculation of 3D traction force from their deformation. With 3D-µTFM, it is shown that MCF10A breast epithelial cells exert defined, reproducible patterns of forces on their microenvironment, which can be both contractile and extensile. Cells switch from a global contractile to extensile behavior as their volume is reduced are further shown. The technique enables the quantitative study of cell mechanobiology with full access to 3D cellular forces while having accurate control over cell morphology and the mechanical conditions of the microenvironment.
Source: Advanced Science
Authors: Laura M. Faure, Manuel Gómez‐González, Ona Baguer, Jordi Comelles, Elena Martínez, Marino Arroyo, Xavier Trepat, Pere Roca‐Cusachs
Institutions: Barcelona Institute of Science and Technology, Institute for Bioengineering of Catalonia, Universitat de Barcelona, Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine, International Center for Numerical Methods in Engineering, Universitat Politècnica de Catalunya, Institució Catalana de Recerca i Estudis Avançats
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,120
Funders: Fundació la Marató de TV3, European Commission, Generalitat de Catalunya, EMBO, Institució Catalana de Recerca i Estudis Avançats, Ministerio de Ciencia e Innovación, Fundación Bancaria Caixa d'Estalvis i Pensions de Barcelona, Horizon 2020 Framework Programme, European Research Council, Horizon 2020, European Regional Development Fund, Agencia Estatal de Investigación, H2020 European Research Council
Awards: G1002562435, G1004203189, Probing and controlling the three-dimensional organoid mechanobiology, G1080425184, G1116193123, G121809054, G1255325603, G1337265928, G1384485580, G1485382716, G1622782223, G1781902864, G2290189609, G2309174360, G2628364946, G2660406951, G2673512141, G2675200210, G2783511746, G3033626195, G3114703784, G3261693824, G3288897321, G3480869486, G3518538256, G3522231704, G3690828678, G4268310340, G434337789, G4526231891, G4529705867, G4937468798, G4944594896, G5080475149, Mechanical control of biological function, G5132640529, G5175525644, G5178665745, G5955501538, G5984434282, G6065495000, G621789379, G6230795418, G683931746, G7061641793, G7084143925, G7425137858, G7434936867, G7530671134, G7533394721, G7613207111, G7685134749, G7731791180, G7762379302, G7976986828, G8368937562, G8684520763, G8880211365, G8920660035, G8935248985, G897484643, G918993045
OpenAlex ID: W4403745912