Year: 2024
Type: preprint
Abstract: This protocol describes a method used to section and stain the intrinsic tongue muscles of the Ts65Dn mouse model of Down syndrome and euploid sibling controls. Unfixed, thin tissue sections are labeled through immunofluorescence staining with antibody detection of laminin to reveal myofiber borders and other extracellular structures. Antibodies to Myosin Heavy Chain isoforms (MyHC) are used to reveal different populations of myofibers. Analysis of stained intrinsic tongue sections contributes to a larger study determining how neuromuscular maturation of the intrinsic tongue is impacted by age and genotype in this mouse model.
Authors: Tiffany J Glass, Kabao Hang
Institution: University of Wisconsin–Madison
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4403752683