Work

Efficient gene disruption in polyploid genome by Cas9–Trex2 fusion protein

Year: 2024

Type: article

Abstract: The fusion of the exonuclease Trex2 with the Cas9 protein significantly enhanced the efficiency of genome editing in hexaploid common wheat, particularly for the simultaneous editing of multiple favorable alleles within a single generation, thereby facilitating genome editing-assisted breeding in polyploid crops.

Source: Journal of Integrative Plant Biology

DOI: 10.1111/jipb.13797

Authors: Wenbo Pan, Chunlei Gao, De Niu, Jinghua Cheng, Jiao Zhang, Xiying Yan, Qiang Long, YaoYao Zhu, Wenjing Sun, Qi Xie, Yuehui He, Xing Wang Deng, Huawei Zhang, Jian Li

Institutions: South China Normal University, Peking University, Chinese Academy of Sciences, State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

Estimated APC paid: $2,730

Funders: Taishan Scholar Foundation of Shandong Province, Natural Science Foundation of Shandong Province

Awards: G391452596, G5220310700, G6812608120

OpenAlex ID: W4403765537

FWCI: 1.385

Cites: 10

Cited by: 12

Related to: 10