Work

Deleterious Effects of Histidine Tagging to the SH3b Cell Wall-Binding Domain on Recombinant Endolysin Activity

Year: 2024

Type: article

Abstract: . Moreover, although recombinant endolysins with a C-terminal 6x histidine tag (His-tag) are often produced and convenient to purify, the deleterious effects of His-tags on antibacterial activity have not been evaluated thoroughly. Recently, we reported that the antibacterial activity of a commercial lysostaphin without a His-tag differed from that of cell-free lysostaphin with a C-terminal His-tag, and lysostaphin also contains a C-terminal SH3b CBD. In this study, we directly compared the effects of His-tags on the antibacterial activities of lysostaphin and several chimeric lysins possessing different SH3b CBDs. We confirmed that antibacterial activity decreased 16.0-32.0-fold after a His-tag was added to the SH3b CBD.

Source: Journal of Microbiology and Biotechnology

DOI: 10.4014/jmb.2408.08003

Authors: Jin-Mi Park, Jun-Hyun Kim, Kang-Seuk Choi, Hyuk-Joon Kwon

Institutions: Seoul National University, Genexine (South Korea), Research Institute for Veterinary Science

Language: English

Topic: Bacteriophages and microbial interactions

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

Open Access status: bronze

OpenAlex ID: W4403820302

FWCI: 0.272

Cites: 28

Cited by: 1

Related to: 10