Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular Dynamics
Year: 2024
Type: article
Abstract: Live-cell imaging generally requires pretreatment with fluorophores to either monitor cellular functions or the dynamics of intracellular processes and structures. We have recently introduced full-field optical coherence tomography for the label-free live-cell imaging of fungi with potential clinical applications for the diagnosis of invasive fungal mold infections. While both the spatial resolution and technical set up of this technology are more likely designed for the histopathological analysis of tissue biopsies, there is to our knowledge no previous work reporting the use of a light interference-based optical technique for direct mycological examination and monitoring of intracellular processes. We describe the first application of dynamic full-field optical transmission tomography (D-FF-OTT) to achieve both high-resolution and live-cell imaging of fungi. First, D-FF-OTT allowed for the precise examination and identification of several elementary structures within a selection of fungal species commonly known to be responsible for invasive fungal infections such as Candida albicans, Aspergillus fumigatus, or Rhizopus arrhizus. Furthermore, D-FF-OTT revealed the intracellular trafficking of organelles and vesicles related to metabolic processes of living fungi, thus opening new perspectives in fast fungal infection diagnostics.
Source: Journal of Fungi
DOI: 10.3390/jof10110741
Authors: Eliott Teston, Marc Sautour, Léa Boulnois, Nicolas Augey, Abdellah Dighab, Christophe Guillet, Dea Garcia-Hermoso, Fanny Lanternier, Marie-Elisabeth Bougnoux, Frédéric Dalle, Louise Basmaciyan, Mathieu Blot, Pierre-Emmanuel Charles, Jean-Pierre Quenot, Bianca Podac, Catherine Neuwirth, Claude Boccara, Martine Boccara, Olivier Thouvenin, Thomas Maldiney
Institutions: Inserm, Université de Bourgogne, Centre de recherche Translationnelle en Médecine moléculaire, Procédés Alimentaires et Microbiologiques, CHU Dijon Bourgogne, Institut Agro Dijon, Centre Hospitalier William Morey, Arts et Métiers, Institut Pasteur, Université Paris Cité, Hôpital Necker-Enfants Malades, Assistance Publique – Hôpitaux de Paris, Centre National de la Recherche Scientifique, Laboratoire Chrono-Environnement, Université Bourgogne Franche-Comté, Université Paris Sciences et Lettres, Institut Langevin, ESPCI Paris, École Normale Supérieure - PSL, Institut de Systématique, Évolution, Biodiversité
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,955
OpenAlex ID: W4403833060