Get started
Work

Cartilage Laser Engraving for Fast-Track Tissue Engineering of Auricular Grafts

Year: 2024
Type: article
Abstract: In this study, the optimal engraving parameters were determined through the analysis of scanning electron microscopy (SEM) data, as follows: a laser power density of 5.5 × 105 W/cm2, an irradiation rate of 0.1 mm/s, a well radius of 60 μm, a distance between well centers of 200 μm, and a number of passes for each well of 20. After 1 week of in vitro cultivation, chondrocytes were located on the surface of the scaffolds, in the sockets and lacunae of decellularized cartilage. When implanted into animals, both cellular and acellular scaffolds were able to support cartilage in-growth and complete regeneration of the defect without clear boundaries with normal tissue. Nevertheless, the scaffolds populated with cells exhibited superior biocompatibility and were not subject to rejection, in contrast to cell-free constructs.
DOI: 10.3390/ijms252111538
Authors: Anastas A. Kisel, Vladimir A. Stepanov, Elena V. Isaeva, Grigory A. Demyashkin, Evgeny I. Isaev, Ekaterina I. Smirnova, Elena M. Yatsenko, Grigoriy V. Afonin, Sergey A. Ivanov, Dmitrii A. Atiakshin, Petr V. Shegay, Andrey D. Kaprin, Ilya D. Klabukov, Denis S. Baranovskii
Institutions: Medical Radiological Research Center, Obninsk Institute for Nuclear Power Engineering, National Research Nuclear University MEPhI, Sechenov University, Peoples' Friendship University of Russia, University of Basel, University Hospital of Basel
Language: English
Subfield: Surgery
Field: Medicine
Open Access status: gold
Estimated APC paid: $3,296
OpenAlex ID: W4403845019
FWCI: 0.762
Cites: 50
Cited by: 2
Related to: 10