Figure 8 from A Database Tool Integrating Genomic and Pharmacologic Data from Adrenocortical Carcinoma Cell Lines, PDX, and Patient Samples
Year: 2024
Type: other
Abstract: The CU-ACC1 cell line is sensitive to temozolomide as it lacks MGMT and is MMR proficient, whereas CU-ACC2 cells are resistant to temozolomide because of MMR deficiency. A, CU-ACC1 and CU-ACC2 do not express MGMT transcripts. Univariate scatterplot of MGMT transcriptional expression levels vs. MGMT gene promoter methylation levels in the ACC cell line dataset. B, MGMT protein expression levels in CU-ACC1, CU-ACC2, NCI-H295R, and SW-13. Proteins were extracted from each cell line, and MGMT expression was assessed by Western blotting. C, The CU-ACC2 cell line is defective in MMR due to lack of expression of the MSH2 gene. Univariate scatter plot of MHS2 transcript levels vs. MSH2 gene copy number in the ACC cell lines data set. D, Dose–response curves of temozolomide in CU-ACC1, CU-ACC2, NCI-H295R, and SW-13. Cell viability was assessed after 72 hours under the indicated drug concentrations by CellTiter-Glo assay.
Authors: Yasuhiro Arakawa, Fathi Elloumi, Sudhir Varma, Prashant Khandagale, Ukhyun Jo, Suresh Kumar, Nitin Roper, William C. Reinhold, Robert W. Robey, Naoko Takebe, Michael M. Gottesman, Craig J. Thomas, Valentina Boeva, Alfredo Berruti, Andrea Abate, Mariangela Tamburello, Sandra Sigala, Constanze Hantel, Isabel Weigand, Margaret E. Wierman, Katja Kiseljak-Vassiliades, Jaydira Del Rivero, Yves Pommier
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4403887363