Work

Study of the mechanisms of colistin heteroresistance in a strain of Enterobacter cloacae by random mutagenesis

Year: 2024

Type: article

Abstract: • Enterobacter presents unique resistance mechanisms in the Enterobacterales order. • Colistin heteroresistance mechanisms were investigated by random mutagenesis. • The arnA gene was knocked out in six colistin-susceptible clones at the same position. • The mcr-9 gene was not involved in colistin resistance in the Enterobacter cloacae complex. • The arnBCADTEF operon plays a major role in colistin resistance in this genus. The Enterobacter cloacae complex belongs to a group in which colistin resistance is not well documented due to their frequent heteroresistance. We investigated the molecular basis of colistin heteroresistance using genome analysis and random mutagenesis in a strain of E. cloacae . The arnA gene was truncated in the six colistin susceptible mutants we obtained. However, the mcr-9 gene was present in these mutants suggesting this gene is not responsible for colistin resistance. Previous studies have highlighted the role of the two-component system PhoPQ and its negative regulator mgrB or the arnBCADTEF operon. Our results confirm the crucial role of this operon.

Source: Journal of Global Antimicrobial Resistance

DOI: 10.1016/j.jgar.2024.10.260

Authors: Romane Milleville, Linda Hadjadj, Jean-Marc Rolain, Sophie Alexandra Baron

Institutions: Aix-Marseille Université, Méditerranée Infection Foundation, Assistance Publique Hôpitaux de Marseille

Language: English

Topic: Antibiotic Resistance in Bacteria

Subfield: Molecular Medicine

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,890