Work

Multi-species cryoEM calibration and workflow verification standard

Year: 2024

Type: article

Abstract: Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical techniques with calibration standards, cryoEM has lacked comprehensive biological test samples. We introduce a cryoEM calibration sample that is a mixture of compatible macromolecules that can be used not only for resolution optimization but also provides multiple reference points for evaluating instrument performance, data quality, and image processing workflows in a single experiment. This combined test specimen provides researchers a reference point for validating their cryoEM pipeline, benchmarking their methodologies, and testing new algorithms.

Source: Acta Crystallographica Section F Structural Biology Communications

DOI: 10.1107/s2053230x24010318

Authors: Daija Bobe, Mykhailo Kopylov, Jessalyn Miller, Aaron P. Owji, Edward T. Eng

Institution: New York Structural Biology Center

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,350

Funders: Simons Foundation, University of Leicester, National Institutes of Health, National Institute of General Medical Sciences