Antioxidant effect and protective effect of Salvia plebeia extract against H2O2-induced oxidative stress in neuronal cell
Year: 2024
Type: article
Abstract: Neuronal cells from oxidative stress are caused by the overproduction of reactive oxygen species or dysfunction of antioxidant system, which are closely associated with neurodegenerative diseases.Salvia plebeia R. has been used as traditional medicine for treating asthma or respiratory symptoms.In this study, the antioxidant activity and protective effect of S. plebeia ethanol extract (SPE) on SH-SY5Y cells induced by oxidative stress were investigated.As a result, the total phenol content was 226.61±19.84mg GAE/g, and total flavonoid content was 46.30±0.71mg QE/g in SPE.SPE showed strong DPPH radical scavenging efficacy at concentrations of 75 and 100 µg/ mL, showing 82.83±6.82 and 89.13±7.60%,respectively.SPE also scavenged ABTS radicals, in particular, 100 µg/mL SPE showed the highest ABTS radical scavenging activity, showing 90.35±0.34%.SPE showed high •OH radical elimination at concentrations of 25, 50, 75, and 100 µg/mL, indicating over 90% scavenging activity.Treatment with hydrogen peroxide (H 2 O 2 , 500 µM) induced neuronal cell death compared to non-treated cells.However, SPE treatment significantly increased cell viability and decreased the amount of lactate dehydrogenase release.Furthermore, total apoptotic cells were decreased by treatment with SPE.These findings suggest that SPE possesses antioxidant properties and neuroprotective effects against oxidative stress and may partially prevent neurodegenerative diseases.
Authors: Yuri Jeong, Seok Hee Han, Ga Yeon Nam, Hyun Young Kim, Ji Hyun Kim, Ah Young Lee, Weon Taek Seo
Institution: Gyeongsang National University
Language: English
Subfield: Biochemistry
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4403954866