Work

Changes in the serum proteome profile of patients with neuroborreliosis, foresters, and patients treated according to ILADS method

Year: 2024

Type: article

Abstract: The aim of study was to evaluate the changes in proteomic profile of human serum induced by the development of tick-borne neuroborreliosis (NB), before/after therapy, patients treated with prolonged multidrug therapy according to ILADS (International Lyme and Associated Diseases Society), and foresters frequently exposed to tick bites. A proteomics approach was used to analyze the expression of proteins in serum of patients and sex/age-matched healthy donors. The analysis was performed using SDS-PAGE/LC-MS/MS (Q-Exactive OrbiTrap mass spectrometer). Obtained results indicated changes in the serum proteome of patients with NB putting attention to the proteins involved mainly in calcium transport/metabolism and signaling molecules that differ patients before and after classic therapy. Moreover, ILADS treated patients have different protein distribution than patients from other groups, what is the consequence of prolonged antibiotic therapy. In the case of foresters, the most important result is the increased β-secretase level. Obtained results may contribute to a better understanding of the mechanism of the development of tick-borne diseases, as well as will allow create new opportunities for its rapid and more effective therapy. However, further studies, on larger patients groups, are needed to apply them in clinical practice. • NB development changes the expression of proteins involved mainly in calcium transport. • In NB patients pro-inflammatory signaling is accompanied by apoptosis/endocytosis. • Foresters have strong changes in their serum proteome as a result of multiple tick bites. • Foresters proteome changes concern on antioxidants and pro-inflammatory proteins.

Source: Microbial Pathogenesis

DOI: 10.1016/j.micpath.2024.107094

Authors: Agnieszka Gęgotek, Elżbieta Skrzydlewska, Monika Groth, Piotr Czupryna, Anna Moniuszko-Malinowska

Institution: Medical University of Białystok

Language: English

Topic: Vector-borne infectious diseases

Subfield: Parasitology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $2,960