Development of Advanced Oral-on-a-Chip: Replicating the Intricate Human Oral Microenvironment
Year: 2024
Type: article
Abstract: models often fail to capture the complexity of these interactions and the multicellular architecture of the oral environment. To address this limitation, we developed an advanced 3D oral-on-a-chip system that mimics the dynamic microenvironment of oral tissues. This system incorporates multiple oral cells into a 3D structure made from natural polymers such as collagen and hyaluronic acid, crosslinked by blood-coagulating factors. Our study revealed that tonsil-resident stem cells are more sensitive to toxic exposure compared to differentiated cells like fibroblasts and endothelial cells. SERPINB2 was identified as a key biomarker of oral toxicity, with significant upregulation observed in tonsil-resident stem cells after exposure to toxins. Based on this, we developed a fluorescence-linked toxicity detection system using SERPINB2, enabling sensitive and quantitative assessments of oral toxicity. This integrated system provides a valuable tool for evaluating the oral toxicity of drug candidates.
DOI: 10.7150/ijbs.104351
Authors: Soo-Rim Kim, Eun-Kyung Min, Choon-Mi Lee, Jin Woo Lee, Chan Hum Park, YunJae Jung, Byung-Chul Oh, Hwa-Yong Lee
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,337
OpenAlex ID: W4403987562