Work

Removal of sequencing adapter contamination improves microbial genome databases

Year: 2024

Type: article

Abstract: Advances in assembling microbial genomes have led to growth of reference genome databases, which have been transformative for applied and basic microbiome research. Here we show that published microbial genome databases from humans, mice, cows, pigs, fish, honeybees, and marine environments contain significant sequencing-adapter contamination that systematically reduces assembly accuracy and contiguousness. By removing the adapter-contaminated ends of contiguous sequences and reassembling MGnify reference genomes, we improve the quality of assemblies in these databases.

Source: BMC Genomics

DOI: 10.1186/s12864-024-10956-1

Authors: Andrew H. Moeller, Brian A. Dillard, Samantha L. Goldman, Madalena V. F. Real, Daniel D. Sprockett

Institutions: Princeton University, Cornell University

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,990

Funders: National Institutes of Health, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases