Work

Antibiofilm efficacy of a calcium silicate-based intracanal medicament against Fusobacterium nucleatum strains

Year: 2024

Type: article

Abstract: This study evaluated the antibiofilm efficacy of a calcium silicate-based intracanal medicament (Bio-C Temp) against Fusobacterium nucleatum ( F. nucleatum ). Dentin slices (n = 88) were inoculated with F. nucleatum biofilms and divided into experimental groups (n = 24 each) treated with Bio-C Temp, triple antibiotic paste (TAP), or Ca(OH) 2 for 1 or 2 weeks. Untreated dentin slices exposed to saline served as a positive control, while sterile dentin slices incubated anaerobically served as a negative control. Bacterial viability was assessed using confocal laser scanning microscopy after staining with LIVE/DEAD ® Bac Light ™ dye. Samples treated with TAP demonstrated the highest percentage of dead bacteria (95%). The antibiofilm effect of TAP and Bio-C Temp significantly increased from Week 1 to Week 2. At Week 2, samples treated with Bio-C Temp showed a significantly higher percentage of dead bacteria (75.25%) than those treated with Ca(OH) 2 (58.65%). TAP showed superior efficacy against F. nucleatum biofilms. After an application time of 2 weeks, the antibiofilm efficacy of Bio-C Temp was superior to that of Ca(OH) 2 .

Source: Scientific Reports

DOI: 10.1038/s41598-024-77130-1

Authors: Hanan Balto, Reem Barakat, Sumaya Basudan, Ghazal Fakeeha, Sarah R. Alharbi, Rahaf Almohareb

Institutions: King Saud University, Princess Nourah bint Abdulrahman University

Language: English

Topic: Endodontics and Root Canal Treatments

Subfield: Oral Surgery

Field: Dentistry

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,590