Acquired blaCfxA-3 carried by a conjugative transposon or duplicated intrinsic blaCME-3 mediates cefiderocol resistance in Elizabethkingia anophelis clinical isolates
Year: 2024
Type: article
Abstract: OBJECTIVES: Elizabethkingia spp. are resistant to multiple antibiotics. This study aimed to determine in vitro and in vivo activities of cefiderocol against Elizabethkingia spp. and to investigate resistance mechanisms. METHODS: Bloodstream isolates were collected from four hospitals. In vitro and in vivo activities were determined using broth microdilution and the wax moth model, respectively. Genome comparison and gene editing were used to confirm the contribution of target genes. Conjugation experiments and serial passage were used to determine transferability and stability, respectively. A MIC of ≤4 mg/L was designated as the susceptibility breakpoint. RESULTS: were 100% and > 90%, respectively, after 10-day serial passage. Cefiderocol failed to rescue moth larvae infected with resistant strains, but removal of resistance mechanisms restored in vivo efficacy. CONCLUSIONS: Cefiderocol was in vitro and in vivo active against Elizabethkingia spp. but resistance may emerge due to the availability, transferability, and/or stability of resistance mechanisms.
Authors: Ya-Sung Yang, Yu-Lin Lee, Yuag-Meng Liu, Chen-Feng Kuo, Mei-Chen Tan, Wei-Cheng Huang, Shu-Yuan Hsu, Yea-Yuan Chang, Hung-Sheng Shang, Shu-Chen Kuo
Institutions: Tri-Service General Hospital, National Defense Medical Center, Chung Shan Medical University Hospital, Chung Shan Medical University, Changhua Christian Hospital, Mackay Memorial Hospital, National Health Research Institutes, Taipei City Hospital, National Yang Ming Chiao Tung University
Language: English
Subfield: Molecular Medicine
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,280
Awards: G1547723851, G1974731686, G2181368520, G3008745174, In Vitro and in Vivo Efficacies of Cefiderocol against Elizabethkingia Species and Investigation of the Mechanisms for Resistance., G3752335598, G4086959021, G4500905393, G477598291, G4873067972, Leveraging Immunopeptidomic and Immunoproteomic to Develop Multi-Epitope Vaccine against Elizabethkingia Anophelis Infection, G5370810467, G6652091631, In-Vitro and In-Vivo Efficacy and Clinical Effectiveness of Vancomycin-Based Therapy on Elizabethkingia Infections and the Mechanism of Action, G6887152956, G7329468443, G7656549468, G7939653657, G7982817548, G80487598, G8638977752
OpenAlex ID: W4404058745