277 Establishment of novel syngeneic mouse gastric cell line models for CLDN18.2 CAR-T therapy
Year: 2024
Type: conference-abstract
Abstract: Background Only a few syngeneic mouse cell line models are available for gastric cancer. Methods We have established a cell line from an autochthonous gastric cancer formed in the stomach of a female genetically-engineered mouse (figure 1). Results This cell line recapitulated morphological and molecular features of human intestinal gastric cancer and readily formed a tumor when injected into syngeneic mice. The cell line overexpressed the isoform 2 of claudin 18, an important molecular therapeutic target in human gastric adenocarcinomas. Anti-mouse CLDN18.2 CAR-T suppressed tumor growth and prolonged survival of mice bearing the syngeneic graft. According to mass spectrometry analysis, the cells had high CLDN18.2 content. Conclusions Thus, we have generated a useful resource for the development of immunotherapeutics. Consent Written informed consent was obtained from the patient for publication of this abstract and any accompanying images. A copy of the written consent is available for review by the Editor of this journal.
Authors: Min Seo, Jin Jeong, Hyeran Kim, Hark Kim
Institution: National Cancer Center
Language: English
Topic: CAR-T cell therapy research
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4404065801