Berberine Mitigates Betel-Nut Induced Hepatocarcinogenesis, Enhances Chemosensitivity to Cisplatin and Reduces Cisplatin- Induced Nephrotoxicity in Mice Exposed to an Aqueous Extract of Betel Nut
Year: 2024
Type: article
Abstract: Drugs and ChemicalsCisplatin(Cisdiammineplatinumdichloride-II),Polyvinyldifluoride (PVDF)(#3010040001) membrane, In situ cell detection Kit, AP(#11684809910), Creatinine AssayKit (#MAK080) and Lactate Dehydrogenase Activity Assay Kit(#MAK066) (SigmaAldrich, St.Louis, USA); Berberine chloride (Abcam, Cambridge, United Kingdom); antibodies against total and phosphorylated Akt(Ser473) (#9272 and #4060), total and phosphorylated p53(Ser15) (#2524and#12571S), total and phosphorylated mTOR (Ser-2448) (#2972S and #2971), PTEN (#9559T), Bcl2 (#3498), CyclinD1 (#2922S), Ki-67 (#12202) and β-actin(#4970S) were from Cell Signaling Technology (Massachusetts, United States).The secondary antibodies specifically designed for detecting rabbit IgG and linked to either alkaline phosphatase (AP) or horseradish peroxidase (HRP) were from Cell Signaling Technology (Massachusetts, United States). Animals All experiments and techniques followed Assam
Source: Pharmacognosy Journal
Institution: Assam University
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4404078601