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A simple, cost-effective, and sustainable method for extraction and quantification of chebulagic acid from Terminalia bellirica (Gaertn.) Roxb. using RP-HPLC: A comparative study

Year: 2024

Type: article

Abstract: Chebulagic acid (CA), a hydrolysable tannin found in Terminalia bellirica (T. bellirica) (Gaertn.) Roxb., has gained prominence as a significant component in Ayurvedic medicine. This study optimized methods for determining CA in T. bellirica , focusing on fruit samples and prioritizing simplicity, cost-effectiveness, less time duration, and sustainability. We evaluated various extraction techniques, including continuous shaking, microwave assisted extraction, and Soxhlet extraction using green solvents. Quantification of CA was conducted using Reverse Phase High-Performance Liquid Chromatography (RP-HPLC), with confirmation achieved via UV–Visible (UV–Vis) spectroscopy, Thin Layer Chromatography (TLC), and Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS). Results indicated that Soxhlet and microwave significant extraction methods, particularly with ethanol as the green solvent. Soxhlet extraction yielded 49,607.73±18.82 μg/mL for fresh fruit and 42,952.53±19.09 μg/mL for dry fruit, while microwave extraction resulted in 43,627.56±113.48 μg/mL for fresh fruit and 40,466.33±130.90 μg/mL for dry fruit respectively. These findings conclude that ethanol as a green solvent in Soxhlet or microwave assisted extraction emerge as most efficient, straightforward, and effective approach for detecting and screening CA in T. bellirica samples.

Source: Green Analytical Chemistry

DOI: 10.1016/j.greeac.2024.100170

Authors: Nisha Nerlekar, Aditya Magdum, Vinod Nandre, Mansingraj Nimbalkar, Padma Dandge

Institution: Shivaji University

Language: English

Topic: Phytochemicals and Medicinal Plants

Subfield: Complementary and alternative medicine

Field: Medicine

Domain: Health Sciences

SDG: Responsible consumption and production

Open Access status: gold

Estimated APC paid: $1,250