Work

Comparison of two methods of extracting bull epididymal spermatozoa

Year: 2024

Type: article

Abstract: /mL in Andromed; the sperm sample was packed in 0.25 mL straws. After cooling for two h at 5 °C, the straws were placed 4 cm above liquid nitrogen for 20 min before transferring them to liquid nitrogen. Sperm motility, viability, reactive oxygen species, membrane integrity and DNA fragmentation were analysed in the fresh samples and again after thawing. The results for all parameters in fresh semen were not different between methods. Although sperm quality was lower in thawed samples than in fresh samples, there was no difference in sperm quality between the two extraction methods in the thawed samples. In conclusion, both methods are useful for the extraction of bull epididymal spermatozoa.

Source: Veterinary and Animal Science

DOI: 10.1016/j.vas.2024.100407

Authors: Ziyad Al-Kass, Sanna Eriksson, Jaana Peippo, Theodoros Ntallaris, Jane M. Morrell

Institutions: University of Mosul, Swedish University of Agricultural Sciences, NordGen

Language: English

Topic: Sperm and Testicular Function

Subfield: Reproductive Medicine

Field: Medicine

Domain: Health Sciences

Open Access status: gold

Estimated APC paid: $2,620

Funders: University of Mosul, Sveriges Lantbruksuniversitet