On-site visual detection of Nipah virus combining a reverse transcription recombinase-aided amplification with a lateral-flow dipstick assay1
Year: 2024
Type: article
Abstract: • The RT-RAA-VF assay developed for the NiV P gene can perform rapid detection of NiV within 20 min at 42°C with high specificity. • This assay is capable of attaining sensitivity to a single copy of NiV RNA transcripts. • This assay effectively avoids false positives caused by aerosol contamination with a sealed disposable nucleic acid visualization test paper device.
Authors: KaiKai Jin, JunJie Zhao, Huanxin Chen, Zimo Zhang, Zengguo Cao, Zanheng Huang, Hao Li, Yongsai Liu, Lisi Ai, Yufei Liu, Changqi Fan, Yuanyuan Li, Pei Huang, Hualei Wang, Haili Zhang
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,000
OpenAlex ID: W4404095542