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Culture-Independent molecular techniques for bacterial detection in bivalves

Year: 2024
Type: article
Abstract: Bivalves, essential to aquatic ecosystems and human consumption, host diverse bacterial communities that traditional culture-based methods struggle to detect, particularly unculturable and fastidious bacteria. This review highlights advancements in culture-independent molecular techniques for bacterial detection in bivalves, focusing on methods such as Polymerase Chain Reaction (PCR), quantitative PCR (qPCR), and next-generation sequencing (NGS), which enable rapid and sensitive identification of diverse bacterial populations . These molecular approaches are instrumental in detecting both pathogenic and symbiotic bacteria , thereby enhancing disease management in aquaculture and safeguarding public health. Comparative insights are provided into the limitations of traditional methods, with molecular techniques offering superior detection of a broader microbial spectrum. Practical applications in aquaculture are discussed through case studies , showcasing these tools’ value in monitoring bacterial diversity and managing disease outbreaks. The review further addresses emerging technologies, such as CRISPR-based diagnostics and portable sequencing, which hold promise for real-time, on-site bacterial monitoring. By promoting efficient and comprehensive bacterial detection , these evolving molecular techniques present forward-looking solutions for sustaining and improving bivalve aquaculture productivity. This review encourages continued research and innovation to address current challenges and harness the full potential of these advanced methodologies.
DOI: 10.1016/j.ejar.2024.11.002
Language: English
Subfield: Immunology
Open Access status: gold
Estimated APC paid: $1,200
OpenAlex ID: W4404141452
FWCI: 1.905
Cites: 77
Cited by: 14
Related to: 10