Bacterial flotillins as destabilizers of phospholipid membranes
Year: 2024
Type: article
Abstract: From archaea to mammals evolutionary conserved flotillins are scaffolding proteins, recognized for their nandomain-segregating activity. Flotillins form basket-like oligomeric architectures on the membrane, based on a conserved secondary structure composition of the monomeric subunits: a membrane-targeting region, an SPFH domain and a coiled-coil "flotillin" domain. In B. subtilis, the two flotillins FloT and FloA are present, localizing mainly in distinct nanodomains and executing multiple cellular functions. We here use deuterium and phosphorus solid-state NMR to monitor the effect of the different flotillins FloT and FloA and their structural components on model membranes. We find a clear disordering effect of FloT and FloA on the membranes reaching the carbon positions in the centre of the membrane. This effect is imposed by the hydrophobic region and the adjacent SPFH domain and, surprisingly, further supported by the membrane-distant flotillin domain. Biological implications of this disordering action are discussed.
Authors: Ana Álvarez-Mena, Estelle Morvan, Denis Martinez, Melanie Berbon, Abigail Savietto Scholz, Axelle Grélard, Sarah Turpin, Erick J. Dufourc, Marc Bramkamp, Birgit Habenstein
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,980
OpenAlex ID: W4404167472