Work

m5C related-regulator-mediated methylation modification patterns and prognostic significance in breast cancer

Year: 2024

Type: article

Abstract: 5-Methylcytosine (m 5 C) is closely associated with cancer. However, the role of m 5 C in breast cancer(BC)remains unclear. This study combined single-cell RNA sequencing (scRNA-Seq) and transcriptomics datasets to screen m 5 C regulators associated with BC progression and analyze their clinical values. Firstly, This study elucidates the mechanisms of the m 5 C landscape and the specific roles of m 5 C regulators in BC patients. we found that the dysregulation of m 5 C regulators with m 5 Cscore play the essential role of the carcinogenesis and progression in epithelial cells and myeloid cells of BC at single cell level. External validation was conducted using an independent scRNA-Seq datasets. Then, three distinct m 5 C modification patterns were identified by transcriptomics datasets. Based on the m 5 C differentially expressed regulators, the m 5 Cscore was constructed, and used to divide patients with BC into high and low m 5 Cscore groups. Patients with a high m 5 Cscore had more abundant immune cell infiltration, stronger antitumor immunity, and better prognoses. Finally, Quantitative real-time (PCR) and immunohistochemistry were used for the in vitro experimental validation, which had extensive prognostic value. In this study, we aimed to assess the expression of m 5 C regulators involved in BC and investigate their correlation with the tumor microenvironment, clinicopathological characteristics, and prognosis of BC. The m 5 C regulators could be used to effectively assess the cell specific regulation prognosis of patients with BC and develop more effective immunotherapy strategies.

Source: Scientific Reports

DOI: 10.1038/s41598-024-77389-4