Rapid Microfluidic Biosensor for Point-of-Care Determination of Rheumatoid Arthritis via Anti-Cyclic Citrullinated Peptide Antibody Detection
Year: 2024
Type: article
Abstract: Rheumatoid arthritis (RA) is a chronic autoimmune disorder that causes extensive damage to multiple organs and tissues and has no known cure. This study introduces a microfluidic detection platform that combines a microfluidic reaction chip with a micro-spectrometer to accurately detect the anti-cyclic citrullinated peptide antibody (anti-CCP Ab) biomarker, commonly associated with arthritis. The surface of the microfluidic reaction chip is functionalized using streptavidin to enable the subsequent immobilization of biotinylated-labeled cyclic citrullinated peptide (biotin–CCP) molecules through a streptavidin–biotin reaction. The modified chip is then exposed to anti-CCP Ab, second antibody conjugated with horseradish peroxidase (HRP) (2nd Ab-HRP), 3,3′,5,5′-tetramethylbenzidine (TMB), and a stop solution. Finally, the concentration of the anti-CCP Ab biomarker is determined by analyzing the optical density (OD) of the colorimetric reaction product at 450 nm using a micro-spectrometer. The detection platform demonstrated a strong correlation (R2 = 0.9966) between OD and anti-CCP Ab concentration. This was based on seven control samples with anti-CCP Ab concentrations ranging from 0.625 to 100 ng/mL. Moreover, for 30 artificial serum samples with unknown anti-CCP Ab concentrations, the biosensor achieves a correlation coefficient of (R2 = 0.9650). The proposed microfluidic detection platform offers a fast and effective method for accurately identifying and quantifying the anti-CCP Ab biomarker. Thus, it offers a valuable tool for the early diagnosis and monitoring of RA and its progression in point-of-care settings.
Source: Biosensors
DOI: 10.3390/bios14110545
Authors: Wei-Yu Tai, To-Lin Chen, Hsing-Meng Wang, Lung-Ming Fu
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,069
Awards: Microfluidic Point-Of-Care Detection Platform for Electrolytes Evaluation of Patients with Chronic Kidney Disease, Kidney Stones and Renal Function Diagnosis, Multifunctional Microfluidic Rapid Amplification and Detection System, Developing an Integrated Microfluidic Immunoassay Platform for Rapid Detection of Peritoneal Dialysate Biomarkers for Early Diagnosis and Prevention of Severe Peritoneal Sclerosis in Peritoneal Dialysis Patients ( II and II i), Development of Biological Contactless Intelligent Real-Time Monitoring System for Pathogens and Drug Residues in Aquatic Environments( I )
OpenAlex ID: W4404240603