Work

Cationic lipid transfection induces nuclear actin filaments

Year: 2024

Type: article

Abstract: Cationic lipids are widely used for gene delivery. Here, we report the transient formation of nuclear actin filaments in mammalian cells transfected with commercially available transfection reagents regardless of the proteins transfected. Readily detectable with phalloidin, nuclear actin ranges from short filaments to a fully developed network. Nuclear actin filaments persist for hours, peak 20 h after transfection, and may be involved in DNA damage repair.

Source: Cytometry Part A

DOI: 10.1002/cyto.a.24903

Authors: Molika Sinha, Maria Kristha Fernandez, Malte Renz

Institutions: Stanford Medicine, Stanford University, Charité - Universitätsmedizin Berlin

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,780