Work

Regulation of autophagy by Rab27B in colorectal cancer

Year: 2024

Type: article

Abstract: Autophagy is a cellular recycling process that is associated with tumor growth, anti-tumor immune responses, and therapy resistance in colorectal cancer (CRC). In this report, we identify the small GTPase Rab27B to control the autophagy process in CRC. Depletion of Rab27B showed an abnormal accumulation of autophagy vesicles and increased autophagy markers in CRC cells, indicating autophagy dysregulation. Image analysis indicated that autophagy flux is blocked at the autophagosome/lysosome fusion step when Rab27B is lost. While Rab27B deficient cells are proficient at growth under 2D in vitro conditions, cell growth was significantly impacted in both in vitro 3D growth and in vivo tumorigenesis studies. Together, these results demonstrate a new role of Rab27B in the autophagy trafficking process in CRC and identify Rab27B as a potential therapeutic target for CRC.

Source: The International Journal of Biochemistry & Cell Biology

DOI: 10.1016/j.biocel.2024.106693

Authors: Sahida Afroz, Ranjan Preet, Vikalp Vishwakarma, Andrew E. Evans, Alexa N. Magstadt, Dan A. Dixon

Institutions: University of Kansas, The University of Kansas Cancer Center

Language: English

Topic: Autophagy in Disease and Therapy

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,650