Efficient directional biosynthesis of isoquercitrin from quercetin by Bacillus subtilis CD-2 and its anti-inflammatory activity
Year: 2024
Type: article
Abstract: Isoquercetin, as the sole product, was directionally biosynthesized from quercetin in a non-aqueous system using Bacillus subtilis CD-2 (1 g/L quercetin), and its structure was identified by LC-MS and NMR analysis. CCK8 assays showed no cytotoxicity and good cell proliferation. The anti-inflammatory experiments demonstrated strong inhibition of NO release, transcriptional downregulation of classical effective cellular factors tumour necrosis factor-α, interleukin-6, interleukin-1β, and transcriptional upregulation of interleukin-10 in LPS-induced RAW264.7 cells. Its stronger anti-inflammatory activity than quercetin provided a reference for modifying the structure of quercetin to obtain more compounds with better pharmacological activities for medical industry.
Source: Natural Product Research
Authors: Ju Han, Jingru Ma, Ruiqi He, Fan Yang, Jingyi Meng, Jiaqi Liu, Fanxing Shi, Jinao Duan, Liangliang Chen, Sen Zhang
Institution: Nanjing University of Chinese Medicine
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4404301713