Enhancement of non-oleaginous green microalgae Ulothrix for bio-fixing CO2 and producing biofuels by ARTP mutagenesis
Year: 2024
Type: article
Abstract: Background Oleaginous green microalgae are often mentioned in algae-based biodiesel industry, but most of them belong to specific gena(Chlorella, Scenedesmus, Botryococcus and Desmodesmus). Thus, the microalgal germplasm resources for biodiesel production are limited. Mutagenesis is regarded as an important technology for expanding germplasm resources. The main purpose of this study is to screen microalgae strains with high carbon dioxide tolerance and high lipid content from mutants derived from indigenous non-oleaginous green microalgae species- Ulothrix SDJZ-17. Results Two mutants with high CO2 tolerance and high lipid content genetic stability were obtained from the mutants by high-throughput screening, named Ulothrix SDJZ-17-A20 and Ulothrix SDJZ-17-A23. In order to evaluate the potential of CO2 fixation and biofuel production, A20 and A23 were cultured under air and 15% CO2 (v/v) conditions, and their wild-type strains (WT) were used as controls. Under the condition of high CO2 concentration, the growth performance and lipid production capacity of mutant strains A20 and A23 were not only significantly better than those of wild strains, but also better than those of their own cultured under air conditions. Among them, A23 obtained the highest LCE (14.79%), Fv/Fm (71.04%) and biomass productivity (81.26 mg L− 1d− 1), while A20 obtained the highest lipid content (22.45% ). Both mutants can be used as candidate strains for CO2 fixation and biofuel production. Conclusion By ARTP mutagenesis with high-throughput screening, the mutants with higher CO2 tolerance, photosynthetic efficiency and lipid productivity can be obtained, even if they are derived from non-oleaginous microalgae, which is of great significance for enriching the energy microalgae germplasm bank, alleviating the global warming and energy crisis.
Institution: Shandong Jianzhu University
Language: English
Field: Energy
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,440
Funder: Shandong Jianzhu University
OpenAlex ID: W4404311203