Work

Phage Display Against 2D Metal–Organic Nanosheets as a New Route to Highly Selective Biomolecular Recognition Surfaces

Year: 2024

Type: article

Abstract: Peptides are important biomarkers for various diseases, however distinguishing specific amino‐acid sequences using artificial receptors remains a major challenge in biomedical sensing. This study introduces a new approach for creating highly selective recognition surfaces using phage display biopanning against metal–organic nanosheets (MONs). Three MONs (ZIF‐7, ZIF‐7‐NH2, and Hf‐BTB‐NH2) are added to a solution containing every possible combination of seven‐residue peptides attached to bacteriophage hosts. The highest affinity peptides for each MON are isolated through successive bio‐panning rounds. Comparison of the surface properties of the MONs and high‐affinity peptides provide useful insights into the relative importance of electrostatic, hydrophobic, and co‐ordination bonding interactions in each system, aiding the design of future MONs. Coating of the Hf‐BTB‐NH2 MONs onto a quartz crystal microbalance (QCM) produced a five‐fold higher signal for phage with the on‐target peptide sequence compared to those with generic sequences. Surface plasmon resonance (SPR) studies produce a 4600‐fold higher equilibrium dissociation constant (KD) for on‐target sequences and are comparable to those of antibodies (KD = 4 x 10−10 m). It is anticipated that insights from the biopanning approach, combined with the highly tunable nature of MONs, will lead to a new generation of highly selective recognition surfaces for use in biomedical sensors.

Source: Small

DOI: 10.1002/smll.202406339