Comparison of the Effects of Antibiotic Sensitivity and Physical Parameters on the Growth of Burkholderia cepacia complex and Burkholderia cenocepacia
Year: 2024
Type: article
Abstract: Burkholderia cepacia complex (Bcc) has been tied to various FDA drug recalls over the past years. It was found that these bacteria can survive on a broad range of molecules in oxygenic and anoxygenic environments as well as sterilized and non-sterilized environments. The main research question focused on how physical requirements and antibiotics can be used to control Bcc and B. cenocepacia growth. Four replicates of TSB tubes that had pH 4, 6, 7, and 8 were inoculated with Bcc and B. cenocepacia and incubated overnight at 4, 20, 25, 37, and 85°C. The transmission readings of the broth cultures were measured to estimate bacterial growth using a Genesys 2 spectrophotometer. The Kirby-Bauer test was performed using Polymyxin, Ticarcillin, Ticarcillin with Clavulanic acid, Penicillin, Ampicillin, Chloramphenicol, Tetracycline, Erythromycin, and Streptomycin. The E-test was performed using gradient strips of Cefiderocol (C) l (0.016-256 mg/L) and Imipenem-relebactam (IR) (0.002/4-32/4 mg/L). The antibiotic dilution test was performed for Chloramphenicol and Tetracycline after observing larger zones of inhibitions with the Kirby-Bauer test. There was no visible growth of Bcc and B cenocepacia at 4°C and 85 °C at any pH and pH 4 across the temperatures. However, subcultures showed bacterial growth the following day. The growth rates increased significantly at 25 and 37°C as well as pH 6 and 7. The average diameters of the zones of inhibitions of PXB, TCC, TIC, C30, and TE30 for Bcc were 1.3, 3.7, 3.1, 2.0, and 1.16 mm and for B. cenocepacia were 0, 1.2, 1.3, 1.1, and 1.6 mm, respectively. Both Bcc and B. cenocepacia were resistant to P10, AM10, E15, and S10. MIC for the E-test of Bcc and B. cenocepacia for IR and C were 0.67 and 10 and 0.88 and 0. 016. mg/L, respectively. MIC and MBC for the dilution test of the C30 and TE30 for Bcc were 1 and 8 and 64 and 128 and B. cenocepacia 8 and 128 and 16 and 128 µg/ml. These bacteria had faster growth rates with no significant difference in their growth under the various temperature and pH conditions used. The research concluded that both Bcc and B. cenocepacia can grow in typical storage conditions such as 4°C and pH 4, without showing any visible signs of growth. This study showed that B. cenocepacia has significantly higher resistance to antibiotics than Bcc. These results are beneficial for developing strategies to prevent Burkholderia cross-contamination in clinical environments.
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4404328475