Work

Repeated Fed-Batch Culture Strategy for the Synthesis of Polyhydroxybutyrate (PHB) Biopolymers from Sugar Cane Juice Using Azotobacter vinelandii

Year: 2024

Type: article

Abstract: via repeated fed-batch fermentation. An agricultural crop, sugar cane, was used as the sole carbon source. Firstly, batch fermentation was investigated considering variations in incubation times (24 h, 48 h, and 96 h). The highest dry cell weight (DCW) and PHAs of 5.15 ± 0.04 g/L and 4.00 ± 0.04 g/L were obtained after 48 h of incubation time. The optimum time obtained was further varied to investigate the effect of the sugar concentrations in the medium. It was found that bacteria could grow very well and produced the highest DCW and PHAs (11.17 ± 0.15 g/L and 8.77 ± 0.06 g/L) when the culture medium with a 100 g/L sugar concentration was added. Later, repeated fed-batch fermentation was carried out to improve productivity. The results obtained revealed that PHA production was increased in the next cycle of the process. Furthermore, the final productivity (0.104 g/L·h) was increased 1.65-fold compared to the first cycle (0.063 g/L·h). Moreover, the culture strategy showed remarkable results, with reductions in both fermentation time and preparation cost.

Source: Polymers

DOI: 10.3390/polym16223156

Authors: Praepilas Dujjanutat, Pakjirat Singhaboot, Pakawadee Kaewkannetra

Institutions: Khon Kaen University, Srinakharinwirot University

Language: English

Topic: biodegradable polymer synthesis and properties

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

SDGs: Zero hunger, Responsible consumption and production, Affordable and clean energy

Open Access status: gold

Estimated APC paid: $3,069