Exactly defined molecular weight poly(ethylene glycol) allows for facile identification of PEGylation sites on proteins
Year: 2024
Type: article
Abstract: PEGylation (the covalent attachment of one or more poly(ethylene glycol) (PEG) units to a therapeutic) is a well-established technique in the pharmaceutical industry to increase blood-residence time and decrease immunogenicity. A challenging aspect of PEGylation is the dispersity of PEGylation agents, which results in batch-to-batch variations and analytical limitations. Herein, we present an approach to overcome these limitations by manufacturing a defined molecular weight (dispersity-free) PEGylation agent. We synthesise a defined molecular weight (Mw), linear 5 kDa methoxy-PEG (mPEG) active ester in an efficient and scalable manner using an iterative liquid-phase approach based on Nanostar Sieving. We then perform a comparative study on the random PEGylation and subsequent characterisation of the protein bovine serum albumin (BSA), using both the defined Mw, dispersity-free mPEG active ester, and a commercially available disperse 5 kDa mPEG active ester. We demonstrate that the defined Mw PEG both allows for facile monitoring of chemical modification reactions during the synthesis of the PEGylation agents, and facilitates straightforward identification of the PEGylated fragments within a PEGylated protein via a simple peptide mapping approach using UPLC-MS. PEGylation, the covalent attachment of one or more poly(ethylene glycol) (PEG) units to a therapeutic, is commonly used in the pharmaceutical industry, but the dispersity of PEGylation agents results in batch-to-batch variations and analytical limitations. Here, the authors present an approach to overcome these limitations by manufacturing a defined molecular weight, dispersity-free, 5 kDa methoxy PEG-succinimidyl propionate (mPEG-SP5135) using Nanostar Sieving.
Source: Nature Communications
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $6,790
OpenAlex ID: W4404339584