Work

Development of a β-lactamase-based aggregation-induced emission lateral flow strip for the detection of clavulanic acid in Milk

Year: 2024

Type: article

Abstract: Lack of biorecognition elements significantly hinders the development of rapid detection methods for clavulanic acid (CA). To address this, we expressed Class A β-lactamases PC1 in vitro and demonstrated its high affinity for CA. Then we investigated the recognition mechanisms of PC1 for CA and identified key contact amino acids: Ser70, Lys73, Ser130, Glu166, and Lys234. Furthermore, PC1 was utilized as a novel biorecognition element to establish a “pseudo-immuno” lateral flow strip (LFS) for CA detection. Aggregation-induced emission fluorescence microspheres (AIE@FM) and biotin-streptavidin (Bio-SA) were integrated to improve the detection performance of PC1-based LFS. Results showed that the sensitivity (cut-off value) of PC1-based AIE(Bio-SA)-LFS was enhanced 2-fold and 4-fold compared to basic AIE@FM-LFS and traditional Au-based LFS, respectively. Eventually, the proposed PC1-based AIE(Bio-SA)-LFS was successfully verified in milk samples with a cut-off value of 20 ng mL −1 . This study provides a powerful tool for on-site CA monitoring for the first time. • β-lactamase PC1 with specific recognition of clavulanic acid was first prepared. • Molecular docking revealed PC1's recognition mechanisms for clavulanic acid. • AIE@FM and biotin-streptavidin (Bio-SA) were integrated to enhance LFS performance. • A PC1-based AIE(Bio-SA)-LFS was first developed to detect clavulanic acid in milk.

Source: Food Chemistry X

DOI: 10.1016/j.fochx.2024.101950