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Protocol for a low-volume, direct analysis urine preparation procedure for non-targeted GC-MS metabolomics

Year: 2024
Type: article
Abstract: We present a low-volume, direct analysis protocol for non-targeted gas chromatography-mass spectrometry (GC-MS) metabolomics, using 100 μL of urine. The steps include sample collection, stock solution preparation, metabolite extraction, two-step derivation with a drying phase, and analysis via two-dimensional GC time-of-flight MS (GCxGC-TOFMS). This protocol improves the efficiency and thoroughness of urinary metabolite analysis, contributing to advancements in metabolomics research, disease diagnosis, and biomarker discovery. For complete details on the use and execution of this protocol, please refer to Olivier et al. 1 • Collection of urine samples with ethical handling and proper storage • Preparation of laboratory and instruments for optimal GC-MS performance • Steps for conducting two-step derivatization are methoximation followed by silylation • Protocol for comprehensive GCxGC-TOFMS analysis for metabolite detection Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. We present a low-volume, direct analysis protocol for non-targeted gas chromatography-mass spectrometry (GC-MS) metabolomics, using 100 μL of urine. The steps include sample collection, stock solution preparation, metabolite extraction, two-step derivation with a drying phase, and analysis via two-dimensional GC time-of-flight MS (GCxGC-TOFMS). This protocol improves the efficiency and thoroughness of urinary metabolite analysis, contributing to advancements in metabolomics research, disease diagnosis, and biomarker discovery.
DOI: 10.1016/j.xpro.2024.103449
Language: English
Open Access status: gold
Estimated APC paid: $1,500
OpenAlex ID: W4404397380
FWCI: 0.232
Cites: 17
Cited by: 2
Related to: 10