Mechanism of Celastrus Orbiculatus Extract Inhibiting the Invasion and Metastasis of Gastric Cancer Cells Through Rac1/limk1/cofilin 1 Pathway
Year: 2024
Type: article
Abstract: Celastrus orbiculatus extract contains various biologically active components which have extensive medicinal value.The present work investigated the inhibitory mechanism of Celastrus orbiculatus extract on the invasion and metastasis of gastric cancer (GC) cells (GCCs), with particular emphasis on the function of the ras-related C3 botulinum toxin substrate 1 (Rac1)/LIM domain kinase 1 (LIMK1)/cofilin1 signalling pathway (SPW) in this process.First, celastrin was extracted from Celastrus orbiculatus using an ultrasonic extraction (ULE) method and detected by high-performance liquid chromatography (HPLC).The human GCC line MGC-803 was then used in the study.The prepared Celastrus orbiculatus samples showed the same chromatographic peak as the celastrol standard.In contrast to the Ctrl group, the cell proliferation rates (CPRs) and cell invasion rates (CIRs) in groups A, B and C were greatly reduced, while the cell apoptosis rates (CARs) and cell migration rates (CMRs) were greatly increased (p < 0.05).In addition, the Rac1/LIMK1/cofilin1 SPW-associated proteins in the Celastrus orbiculatus treatment groups were strongly downshifted compared to the Ctrl group (p < 0.05).Meanwhile, there was a continuous downward trend with increased DC and time.Celastrus orbiculatus extract, by regulating the Rac1/LIMK1/cofilin1 SPW, suppressed proliferation, invasion and migration of GCCs while facilitating their apoptosis.This discovery provided important evidence for the potential use of Celastrus orbiculatus extract in treating GC. RezumatExtractul de Celastrus orbiculatus conține multiple componente biologic active cu efecte terapeutice.Studiul a investigat mecanismul inhibitor al extractului de Celastrus orbiculatus asupra invaziei și metastazelor celulelor canceroase gastrice (GCC), cu accent deosebit pe funcția substratului 1 (Rac1)/LIM al toxinei botulinice C3 legat de ras 1 (LIMK1)/calea de semnalizare cofilin1 (SPW) în acest proces.În primul rând, celastrin a fost extras din Celastrus Orbiculatus folosind o metodă de extracție cu ultrasunete (ULE) și identificat prin cromatografie lichidă de înaltă performanță (HPLC).Linia GCC umană MGC-803 a fost utilizată în studiu.Probele au prezentat același vârf cromatografic ca standardul de celastrol.Spre deosebire de grupul Ctrl, ratele de proliferare celulară (CPR) și ratele de invazie celulară (CIR) din grupurile A, B și C au fost mult reduse, în timp ce ratele de apoptoză celulară (CAR) și ratele de migrare celulară (CMR) au fost mult crescute (p < 0,05).În plus, proteinele asociate cu Rac1/LIMK1/cofilin1 SPW din grupurile de tratament cu Celastrus orbiculatus au fost puternic reduse în comparație cu grupul Ctrl (p < 0,05).Extractul de Celastrus orbiculatus, prin reglarea SPW Rac1/LIMK1/cofilin1, a suprimat proliferarea, invazia și migrarea GCC facilitând, în același timp, apoptoza acestora.
Source: FARMACIA
Author: RUI HUANG
Institution: Mudanjiang Medical University
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4404419580