Microscale maps of bursting dynamics across human hippocampal slices from patients with epilepsy
Year: 2025
Type: article
Abstract: How seizures begin at the level of microscopic neuronal circuits remains unknown. Advancements in high-density CMOS-based microelectrode arrays can be harnessed to study neuronal network activity with unprecedented spatial and temporal resolution. We use high-density electrophysiology recordings to probe the network activity of human hippocampal brain slices from six patients with mesial temporal lobe epilepsy. Two slices from the dentate gyrus exhibited epileptiform activity in the presence of low magnesium media with kainic acid. Both slices exhibit network oscillations indicative of a reciprocally connected circuit, which is unexpected under normal physiological conditions. Future studies may apply this approach to elucidate the network signals that underlie seizure initiation.
Source: Journal of Neurophysiology
Authors: Matthew A.T. Elliott, John P. Andrews, Tjitse van der Molen, Jinghui Geng, Alex Spaeth, Anna Toledo, Kateryna Voitiuk, Cordero Core, Thomas Gillespie, Ari Sinervo, David F. Parks, Ash Robbins, Daniel Solís, Edward F. Chang, Tomasz Jan Nowakowski, Mircea Teodorescu, David Haussler, Tal Sharf
Language: English
Subfield: Cognitive Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: hybrid
Funders: Schmidt Futures, National Science Foundation, University of California, Santa Cruz, University of Washington, National Institutes of Health, University of California, San Diego, University of California, San Francisco, Office of the President, University of California, National Institute of Mental Health, National Human Genome Research Institute, National Institute of General Medical Sciences, National Institute of Neurological Disorders and Stroke
Awards: G1754555162, NSF2026: EAGER: The evolution and diversity of the human brain, Machine Learning Augmented Discovery of AAV Capsids for Cell Type Specific Access into Human Neurons and Glia, NINDS Research Education Programs for Residents and Fellows in Neurology, Neurosurgery, Neuropathology, Neuroradiology and Emergency Medicine (R25), RECODE-ing the Logic of Ectodermal Organoid Patterning via Combinatorial Morphogen Treatments and Longitudinal Monitoring, IRACDA at UCSC and CSUMB, Dissecting the role of NOTCH2NL genes in human brain development and neurological disorders associated with chromosome 1q21.1 distal duplications and deletions., UCSC Graduate Program in Genome Sciences, Developmental Timing During Cortical Development, Center for Live Cell Genomics
OpenAlex ID: W4417283914