Work

Recent advances in solid‐state nuclear magnetic resonance studies on membrane fusion proteins

Year: 2024

Type: article

Abstract: Membrane fusion is an essential biological process that merges two separate lipid bilayers into a whole one. Membrane fusion proteins facilitate this process by bringing lipid bilayers in close proximity to reduce the repulsive energy between membranes. Along with their interactions with membranes, the structures and dynamics of membrane fusion proteins are key to elucidating the mechanisms of membrane fusion. Solid-state NMR (SSNMR) spectroscopy has unique advantages in determining the structures and dynamics of membrane fusion proteins in their membrane-bound states. It has been extensively applied to reveal conformational changes in intermediate states of viral membrane fusion proteins and to characterize the critical lipid-membrane interactions that drive the fusion process. In this review, we summarize recent advancements in SSNMR techniques for studying membrane fusion proteins and their applications in elucidating the mechanisms of membrane fusion.

Source: FEBS Journal

DOI: 10.1111/febs.17313

Authors: Lifen Zheng, Shenlin Wang

Institutions: East China University of Science and Technology, State Key Laboratory of Bioreactor Engineering

Language: English

Topic: Lipid Membrane Structure and Behavior

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Natural Science Foundation of China, Science and Technology Commission of Shanghai Municipality, Fundamental Research Funds for the Central Universities

Awards: G6677217420, G8376376964