Beginner’s guide to investigating protein: DNA interactions using electrophoretic mobility shift assays (EMSAs)
Year: 2024
Type: article
Abstract: This article describes how DNA molecules typically migrate through a gel/matrix. It describes the materials required to perform electrophoretic mobility shift assays (EMSAs), the methods used to set up a reaction and components that require optimization. It also shines light on the applications of EMSA, including appropriate controls that can make it more interpretive and alternatives to EMSA if the user requires more specialization.
Source: The Biochemist
DOI: 10.1042/bio_2023_125
Author: Shravanti Krishna Suresh
Institution: Mammoth Biosciences (United States)
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4404484438