Work

Glucose-1-phosphate thymidylyltransferase promotes the production of 3- O -α-mycarosylerythronolide B in Streptomyces coelicolor

Year: 2024

Type: article

Abstract: AIMS: The main objective of this study was to produce erythronolide B (EB) and 3-O-α-mycarosylerythronolide B (MEB) in Streptomyces coelicolor and enhance the MEB production by expressing the glucose-1-phosphate thymidylyltransferase (RfbA). METHODS AND RESULTS: We expressed eryF and eryB genes (eryBII, eryBIII, eryBIV, eryBV, eryBVI, and eryBVII) to produce EB and MEB. The expression was confirmed by quantitative real-time polymerase chain reaction. Furthermore, the MEB's production was improved by more than 100-fold by expressing an enzyme, RfbA, which is absent from the erythromycin gene cluster, to promote the biosynthesis of TDP-L-mycarose. We discuss the feasibility of alternative Streptomyces species for erythromycin production based on the presence or absence of RfbA. CONCLUSIONS: The RbfA enzyme from Saccharopolyspora erythraea was expressed in S. coelicolor M1152 along with the MEB biosynthesis pathway, resulting in a large increase in MEB production (>100-fold).

Source: Journal of Applied Microbiology

DOI: 10.1093/jambio/lxae291

Authors: Hong Gao, Swen Langer, Tony Larson, Matthew A Gregory, Margaret C M Smith

Institutions: University of York, Isomerase Therapeutics (United Kingdom)

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

Open Access status: hybrid

Estimated APC paid: $3,844

Funders: Yorkshire Forward, Biotechnology and Biological Sciences Research Council