Temperature tactics: Targeting acetate or methane production in autotrophic H2/CO2 conversion with mixed cultures
Year: 2024
Type: article
Abstract: Ex situ biomethanation is a promising technology that combines the concepts of Power-to-X and carbon capture and utilization (CCU) by employing microorganisms to convert hydrogen (H 2 ) and carbon dioxide (CO 2 ) into biomethane (CH 4 ). Mesophilic (37°C) and thermophilic (50°C) ex situ biomethanation processes in continuous stirred tank reactors (CSTRs), were evaluated for the first time under a high continuous H 2 /CO 2 supply. Each process was evaluated with and without the addition of minerals and vitamins. In all thermophilic reactors, methanogenesis was favored, achieving stable methane yields of 110 ± 8 %. Additionally, the addition of minerals resulted in a 67 % increase in H 2 consumption rates. In contrast, mesophilic reactors showed low CH 4 yields (7.5 %) and acetogenesis as the dominant pathway, with acetate concentrations reaching up to 7.6 ± 2 g L −1 . The effect of free acetic acid (FAA), a rarely considered concept based on the correlation between pH and acetic acid concentration, was also tested on thermophilic ex situ CSTRs. A clear negative correlation was demonstrated between FAA concentration and the CH 4 productivity, with a 50 % inhibition of CH 4 productivity at 0.062 g L −1 of FAA. • The biomethanation process was evaluated under a high continuous flow of H 2 /CO 2 • Acetogenesis was favored in mesophilic bioreactors under high H 2 partial pressures. • Methanogenesis was favored in thermophilic bioreactors under high H 2 partial pressures. • Free acetic acid is a critical inhibitor in thermophilic biomethanation processes.
Source: Biochemical Engineering Journal
Institution: Aarhus University
Language: English
Subfield: Building and Construction
Field: Engineering
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,700
Awards: G6555865878, G7780119528
OpenAlex ID: W4404516521