First report of contagious caprine pleuropneumonia (CCPP) in Bangladeshi goats: Seroprevalence, risk factors and molecular detection from lung samples
Year: 2024
Type: article
Abstract: Background and objectiveContagious caprine pleuropneumonia (CCPP) is an extremely contagious mycoplasmal respiratory infection affecting mainly goats and sheep caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp). The disease is recognized for its increased death, high infection rates and substantial economic repercussions in the global livestock industry. So far, no information is available both on the serological or molecular identification of Mccp in Bangladesh. The objective of this study was to determine the seroprevalence of CCPP and associated risk factors in goats of Bangladesh, as well as to molecularly identify the causal agent (Mccp) for the first time in this country.Materials and MethodsFrom July 2022 to June 2023, 402 goat serum samples were randomly obtained to determine seroprevalence, and 90 clinically suspected lung samples were collected for molecular confirmation. Risk factors were assessed by interviewing goat owners using a questionnaire. A commercially available cELISA kit was applied for screening blood serum for anti-CCPP antibodies and PCR for Mccp detection. Potential risk factors were identified using a univariate logistic regression followed by multivariate logistic regression model. The 16S rRNA gene was used to identify Mycoplasma mycoides cluster (Mmc), and the Mccp-specific gene was used to identify Mycoplasma capricolum subsp. capripneumoniae (Mccp) in suspected lung samples.ResultsOut of 402 samples, there were 29 CCPP-positive serum samples were found. The overall seroprevalence was 7.21% (95% CI: 1.90-12.53). The associated risk factors for the disease were animal age (>18 months; OR: 2.14, 95% CI: 0.92-4.98), sex (Female; OR: 5.80, 95% CI: 1.70-19.69), flock size (Large; OR: 6.28, 95% CI: 1.17-35.74), and body condition scores (Poor; OR: 5.58, 95% CI: 1.36-22.92). Mccp were confirmed molecularly in overall 26.67% (n=90) samples by PCR.ConclusionThis study confirms CCPP in Bangladeshi goats for the first time using serological and molecular methods (PCR). However, vaccinations and using the right antimicrobials can limit the detrimental effects of CCPP in goat populations and prevent economic losses.
Source: Heliyon
Authors: Md Habibur Rahman, Md Shahin Alam, Md Zulfekar Ali, Md Nurul Haque, Sonia Akther, Sadek Ahmed
Institution: Bangladesh Livestock Research Institute
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,100
OpenAlex ID: W4404516725