Work

Ultrasound-modulation assisted multiphoton imaging

Year: 2024

Type: article

Abstract: Two-photon microscopy provides sectioned excitation, but in practical settings, it often suffers from contrast limitations. Here, we report the observation of a strong acousto-optic modulation of two-photon excited fluorescence. Harnessing this effect yields enhanced image detail and contrast and improves optical sectioning in deep brain tissue in vivo. Ultrasound-modulation assisted multiphoton imaging (UMAMI) is compatible with standard multiphoton microscopes, without requiring changes to the optical path or image acquisition parameters.

Source: Advanced Photonics

DOI: 10.1117/1.ap.6.6.066003

Authors: Ezra Guralnik, Behnam Tayebi, Yi Yuan, Justin Little, Michal Balberg, Shy Shoham

Institutions: NYU Langone Health, New York University, Yanshan University, Holon Institute of Technology

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

OpenAlex ID: W4404519979

FWCI: 0.182

Cites: 20

Cited by: 1

Related to: 10