Work

Adenine Base Editing (ABE) Screening of Blood Pressure (BP) and Coronary Artery Disease (CAD)-Associated Variants in Endothelial Cells v1

Year: 2024

Type: preprint

Abstract: This study developed a pooled Adenine Base Editing (ABE) screen to assess the functional impact of genetic variants linked to blood pressure (BP) traits and coronary artery disease (CAD) in a human endothelial cell line (TeloHAEC). Twelve loci identified through Genome-wide association studies (GWAS) were analyzed, including variants in strong linkage disequilibrium (r² ≥ 0.8) across multiple ancestries and fine-mapped (PIP > 0.1) in the UK Biobank. A gRNA library targeting 1,271 variants was designed, incorporating positional effects and nearby genetic variations. This platform enables high-throughput analysis of BP- and CAD-associated variants.

DOI: 10.17504/protocols.io.261geroqwl47/v1

Authors: Estelle Lecluze, Hicham Belhaj, Candice Diaz, Chao-Sheng Lo, Ken Sin Lo, Guillaume Lettre

Language: English

Topic: Genetic Associations and Epidemiology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4404520722

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