MiR-124-3p/EIF3B Regulates Host Cell Apoptosis Induced by Chlamydia psittaci Through PI3K/AKT Signaling Pathway
Year: 2024
Type: article
Abstract: Chlamydia psittaci is a zoonotic pathogen known to cause respiratory diseases in humans. Chlamydia infections are closely associated with apoptosis, in which microRNAs (miRNAs) play regulatory roles. Herein, we demonstrated that C. psittaci infection induces apoptosis in human bronchial epithelial (HBE) cells and investigated regulatory mechanism involving miR-124-3p and the PI3K/AKT signaling pathway. Following C. psittaci infection in HBE cells, we observed an elevated HBE cell apoptosis, accompanied by upregulation of miR-124-3p levels. Mechanistically, we identified EIF3B as a novel target gene of miR-124-3p, supported by the inverse correlation of their mRNA expressions. MiR-124-3p inhibitor reduced apoptosis induced by C. psittaci, increased the replication of C. psittaci, and inhibited PI3K/AKT activation, whereas miR-124-3p mimic produced opposite effects, and transfection with EIF3B siRNA reversed the effects of miR-124-3p inhibitor. Our findings suggest that miR-124-3p targeting EIF3B promotes apoptosis in C. psittaci-infected HBE cells through activation of the PI3K/AKT signaling pathway.
Authors: Ting Tong, Yunfei Li, You Zhou, Xindian Zeng, Cui Xiao, Saihong Cao, Chuan Wang, Zhongyu Li, Zhou Zhou, Qinqin Bai, Shenghua Chen, Shuwu Yan, Lili Chen
Institution: University of South China
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: bronze
Awards: G1773403982, G2274936791, G5655233076
OpenAlex ID: W4404521809